Journal
PHARMANUTRITION
Volume 14, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.phanu.2020.100223
Keywords
Aronia juice; Lipid accumulation; Arrdc3; Sirt2; Ucp1
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Background: Obesity is a serious risk factor in health, and it is helpful for dietary supplements to prevent it. Aronia berries have various beneficial effects on health including amelioration of type 2 diabetes, obesity and hypertension. This study examined mechanistically the effects of aronia juice on the white adipose tissues in normal and obesity model mice. Methods: C57BL/6 J mice and KKAy mice were fed on a normal diet for 28 days. During this feeding period, the study group was administered with aronia juice via a water bottle, while the control group was administered with tap water. Furthermore, gene expression and mass spectrometric analyses were performed. Results: In both the mouse strains, the size of adipose cells in the study group was significantly smaller than that in the control group. In the study group, expression in the white adipose tissues of Arrdc3 mRNA was suppressed, whereas that of Sirt2 and Ucp1 mRNA was enhanced. In 3T3-L1 cells, non-cytotoxic levels of aronia juice inhibited the lipid accumulation and Arrdc3 mRNA expression. Using reverse-phase liquid chromatography, compounds suppressing Arrdc3 expression in 3T3-L1 cells were isolated from aronia juice. Mass spectrometric analysis suggested that these compounds are vicenin-2 and quercetin-diglycoside. Conclusion: The results suggest that aronia juice inhibits lipid accumulation in white adipose tissues by suppressing Arrdc3 mRNA expression in adipocytes.
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