Journal
MITOCHONDRIAL DNA PART B-RESOURCES
Volume 5, Issue 4, Pages 3819-3820Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/23802359.2020.1840935
Keywords
Complete chloroplast genome; Stephania tetrandra; Menispermaceae
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Funding
- Project of Chinese Medicine from Health Commission of Jiangxi Province [2016A046]
- Scientific Research Foundation for the First-class Discipline of Chinese Medicine Program of Jiangxi University of Traditional Chinese Medicine [JXSYLXK-ZHYAO028]
- Traditional Chinese Medicine Public Health Special Project [201207002]
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The complete chloroplast genome of Stephania tetrandra was sequenced and assembled for the first time. The chloroplast genome is 159,974 bp in length, containing a large single-copy (LSC) region of 90,539 bp and a small single-copy region (SSC) of 20,735 bp, separated by a pair of inverted repeats (IRs) of 24,350 bp. The genome contains 113 unique genes, including 79 protein-coding genes (PCGs), 30 tRNA genes, and four rRNA genes. Among them, 15 genes have one intron each and three genes contain two introns. The overall GC content is 37.8%, while the corresponding values of LSC, SSC, and IR regions are 35.8, 32.4, and 43.7%, respectively. Phylogenetic analysis showed that S. tetrandra is more closely related to the clade of two species within Stephania, providing new insight into the evolution of Menispermaceae.
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