3.9 Article

Inhibitory and inductive effects of 4- or 5-methyl-2-mercaptobenzimida-zole, thyrotoxic and hepatotoxic rubber antioxidants, on several forms of cytochrome P450 in primary cultured rat and human hepatocytes

Journal

TOXICOLOGY REPORTS
Volume 7, Issue -, Pages 979-985

Publisher

ELSEVIER
DOI: 10.1016/j.toxrep.2020.08.003

Keywords

Benzimidazole; Cytochrome P450; Drug-metabolizing activity; Hepatocyte; Primary culture

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Funding

  1. Health and Labor Science Research Grant from the Ministry of Health, Labor and Welfare in Japan

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Effects of 4-methyl-2-mercaptobenzimidazole (4-MeMBI) and 5-methyl -2mercaptobenzimidazole (5-MeMBI) on cytochrome P450 (CYP) activity were examined in primary cultured rat hepatocytes. Hepatocytes from male Wistar rats were cultured in the presence of 4-MeMBI or 5-MeMBI (0-400 mu M), and the activity of CYPs 3A2/4 (48 and 96 h) and 1A1/2 (48 h) was determined by measuring the activity of testosterone 68-hydroxylation and 7-ethoxyresorufin O-deethylation, respectively. As a result, 4-MeMBI and 5-MeMBI (>12.5 mu M) inhibited CYP3A2 activity. On the other hand, 4-MeMBI (>25 mu M) and 5-MeMBI (>100 mu M) induced CYP1A1/2 activity, being consistent with the previous in vivo results. In a comparative metabolism study using primary cultured human hepatocytes from two Caucasian donors, 4-MeMBI and 5-MeMBI induced the activity of CYPs 3A4 and 1A1/2 with individual variability. It was concluded from these results that 4-MeMBI, 5-MeMBI and MBI caused inhibition of CYP3A2 activity in primary cultured rat hepatocytes, suggesting their potential for metabolic drug drug interactions. Primary cultured rat and human hepatocytes were considered to be useful for the evaluation of effects of the benzimidazole compounds on their inducibility and inhibitory activities of cytochrome P450 forms.

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