Journal
BRAZILIAN JOURNAL OF POULTRY SCIENCE
Volume 22, Issue 4, Pages -Publisher
FACTA-FUNDACIO ARNCO CIENCIA TECNOLOGIA AVICOLAS
DOI: 10.1590/1806-9061-2019-1128
Keywords
Goose; PEPCK; Sequence characteristics; Hepatic steatosis; Primary hepatocytes
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Funding
- China Agricultural Research System [CARS-42-4]
- Project of National Science and Technology Plan for the Rural Development in China [2015BAD03B06]
- Key Technology Support Program of Sichuan Province [2016NYZ0044]
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Over-accumulation of triglycerides (TGs) in goose hepatocytes leads to the formation of fatty acid liver. Phosphoenolpyruvate carboxylase kinase 1 (PEPCK) is regarded as the rate-limiting enzyme for gluconeogenesis, and there is evidence that PEPCK is involved in regulating hepatic glucolipid metabolism. Hence, we proposed that PEPCK may have a role in goose hepatic steatosis. To test our hypothesis, the present study was conducted to firstly determine the sequence characteristics of goose PEPCK and then to explore its role in overfeeding-induced fatty liver. Our results showed that goose PEPCK encodes a 622-amino-acids protein that contains highly conserved oxaloacetate-binding domain, kinase-1 and kinase-2 motifs. PEPCK had higher mRNA levels in goose liver, and overfeeding markedly increased its expression in livers of both Sichuan White and Landes geese (p<0.05). Besides, expression of PEPCK was positively correlated with hepatic TG levels as well as plasma glucose and insulin concentrations. Additionally, in cultured goose primary hepatocyte, treatment with either oleic acid (0.8, 1.2 or 1.6 mM) or linoleic acid (0.125 or 0.25 mM) significantly (p<0.05) enhanced the expression of PEPCK. Taken together, these data suggested a role for PEPCK in the occurrence of overfeeding-induced goose hepatic steatosis.
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