Journal
ACS APPLIED BIO MATERIALS
Volume 3, Issue 5, Pages 3430-3439Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsabm.0c00419
Keywords
wound dressing; electrospinning; PCL; thymol; chlorohexidine; Staphylococcus aureus
Funding
- Spanish Ministry of Economy and Competitiveness [CTQ2014-52384-R]
- EU [ERC-2013-CoG-614715]
- Instituto de Salud Carlos III (Spain)
- European Regional Development Fund
- FPI program [BES-2015-073735]
- Spanish government
- Miguel Servet Program (Instituto de Salud Carlos III) [MS19/00092]
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Due to the prevalence of antimicrobial resistant pathogens, natural products with long-term antimicrobial activities are considered as potential alternatives. In this work, polycaprolactone (PCL) electrospun fibers with mean diameters around 299 nm and loaded with 14.92 +/- 1.31% w/w thymol (THY) were synthesized. The mats had appropriate elongation at break (74.4 +/- 9.5%) and tensile strength (3.0 +/- 0.5 MPa) to be potentially used as wound dressing materials. In vivo studies were performed using eight to ten week-old male SKH1 hairless mice. The infection progression was evaluated through a semiquantitative method and quantitative polymerase chain reaction. The analyses of postmortem samples indicated that THY-loaded PCL fibers acted as inhibitors of Staphylococcus aureus ATCC 25923 strain growth being as efficient as chlorhexidine (CLXD). Histopathological and immunohistochemical studies showed that the PCL-THY-treated wounds were almost free of an inflammatory reaction. Therefore, wound dressings containing natural compounds can prevent infection and promote wound healing and prompt regeneration.
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