Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 12, Issue 46, Pages 51999-52007Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.0c15669
Keywords
nanoflowers; enzyme; laccase; biosensor; microfluidics
Funding
- Australia Research Council [DP 200101106]
- Government of South Australia
- Australian Nanotechnology Network, through the Overseas Travel Fellowship program
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The fabrication of hybrid protein-Cu-3(PO4)(2) nanoflowers (NFs) via an intermediate toroidal structure is dramatically accelerated under shear using a vortex fluidic device (VFD), which possesses a rapidly rotating angled tube. As-prepared laccase NFs (LNFs) exhibit approximate to 1.8-fold increase in catalytic activity compared to free laccase under diffusion control, which is further enhanced by approximate to 2.9-fold for the catalysis under shear in the VFD. A new LNF immobilization platform, LNF@silica incorporated in a VFD tube, was subsequently developed by mixing the LNFs for 15 min with silica hydrogel resulting in gelation along the VFD tube surface. The resulting LNFs@silica coating is highly stable and reusable, which allows a dramatic 16-fold enhancement in catalytic rates relative to LNF@silica inside glass vials. Ultraviolet-visible spectroscopy-based real-time monitoring within the LNFs@silica-coated tube reveals good stability of the flow processing. The results demonstrate the utility of the VFD microfluidic platform, further highlighting its ability to control chemical and enzymatic processes.
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