Journal
MITOCHONDRION
Volume 55, Issue -, Pages 100-110Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.mito.2020.09.006
Keywords
Biomarker; Exosome; Mitochondria; mitochondrial DNA; NUMT
Categories
Funding
- University of Kansas Alzheimer's Disease Center [P30AG035982]
- Mabel Woodyard Fellowship award
- NIH/NIGMS COBRE grant [P20GM104936]
- NIH [1S10RR027564]
Ask authors/readers for more resources
We screened cell line and plasma-derived exosomes for molecules that localize to mitochondria or that reflect mitochondrial integrity. SH-SY5Y cell-derived exosomes contained humanin, citrate synthase, and fibroblast growth factor 21 protein, and plasma-derived exosomes contained humanin, voltage-dependent anion-selective channel 1, and transcription factor A protein. Nuclear mitochondrial (NUMT) DNA complicated analyses of mitochondrial DNA (mtDNA), which otherwise suggested exosomes contain at most very low amounts of extended mtDNA sequences but likely contain degraded pieces of mtDNA. Cell and plasma-derived exosomes contained several mtDNA-derived mRNA sequences, including those for ND2, CO2, and humanin. These results can guide exosome-focused, mitochondria-pertinent biomarker development.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available