Journal
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
Volume 46, Issue 2, Pages 93-105Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/10408347.2014.980775
Keywords
tandem mass-spectrometry; Analytical method development; matrix effects; biomonitoring; biological analysis
Categories
Funding
- American Recovery and Reinvestment Act under NIH [5RC1ES01829902]
- National Children's Study [HHSN267200700007C]
- PBDE Body Burdens, House Dust Concentrations, and Associations with Thyroid Hormones under NIH [1R21ES019697-01]
- Emory Parkinson's disease Collaborative Environmental Research Center under NIEHS [P01 ES016731]
- HERCULES: Health and Exposome Research Center at Emory University under NIEHS [1P30ES019776-01A1]
- Graduate and Postdoctoral Training in Toxicology NIH grant [T32 ES012870]
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The ability to quantify levels of target analytes in biological samples accurately and precisely in biomonitoring involves the use of highly sensitive and selective instrumentation such as tandem mass spectrometers and a thorough understanding of highly variable matrix effects. Typically, matrix effects are caused by co-eluting matrix components that alter the ionization of target analytes as well as the chromatographic response of target analytes, leading to reduced or increased sensitivity of the analysis. Thus, before the desired accuracy and precision standards of laboratory data are achieved, these effects must be characterized and controlled. Here we present our review and observations of matrix effects encountered during the validation and implementation of tandem mass spectrometry-based analytical methods. We also provide systematic, comprehensive laboratory strategies needed to control challenges posed by matrix effects in order to ensure delivery of the most accurate data for biomonitoring studies assessing exposure to environmental toxicants.
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