Journal
CRITICAL REVIEWS IN ANALYTICAL CHEMISTRY
Volume 45, Issue 2, Pages 106-111Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/10408347.2014.892820
Keywords
calibration; reference materials; Kjeldahl methods; clinical chemistry; total protein
Categories
Ask authors/readers for more resources
We found previously that albumin-calibrated total protein in certified reference materials causes unacceptable positive bias in analysis of human sera. The simplest way to cure this defect is the use of human-based serum/plasma standards calibrated by the Kjeldahl method. Such standards, commutative with serum samples, will compensate for bias caused by lipids and bilirubin in most human sera. To find a suitable primary reference procedure for total protein in reference materials, we reviewed Kjeldahl methods adopted by laboratory medicine. We found two methods recommended for total protein in human samples: an indirect analysis based on total Kjeldahl nitrogen corrected for its nonprotein nitrogen and a direct analysis made on isolated protein precipitates. The methods found will be assessed in a subsequent article.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available