Journal
CELL REPORTS MEDICINE
Volume 1, Issue 9, Pages -Publisher
CELL PRESS
DOI: 10.1016/j.xcrm.2020.100161
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Funding
- National Natural Science Foundation of China [61971255, 81872368]
- Shenzhen Science and Technology Innovation Committee [KQJSCX20180327143623167, JCYJ20180508152130899, GJHZ20180420180754917]
- Shenzhen Development and Reform Commission Subject Construction Project [[2017]1434]
- State Key Lab Construction Project [GuoKeFaJi [2018]38]
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Current organoid technologies require intensive manual manipulation and lack uniformity in organoid size and cell composition. We present here an automated organoid platform that generates uniform organoid precursors in high-throughput. This is achieved by templating from monodisperse Matrigel droplets and sequentially delivering them into wells using a synchronized microfluidic droplet printer. Each droplet encapsulates a certain number of cells (e.g., 1,500 cells), which statistically represent the heterogeneous cell population in a tumor section. The system produces >400-mu m organoids within 1 week with both inter-organoid homogeneity and inter-patient heterogeneity. This enables automated organoid printing to obtain one organoid per well. The organoids recapitulate 97% gene mutations in the parental tumor and reflect the patient-to-patient variation in drug response and sensitivity, from which we obtained more than 80% accuracy among the 21 patients investigated. This organoid platform is anticipated to fulfill the personalized medicine goal of 1-week high-throughput screening for cancer patients.
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