Journal
BIOFILM
Volume 2, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.bioflm.2019.100010
Keywords
Biofilm; Reproducibility; Guidelines; Microplate; Spectrophotometry; Fluorometry
Categories
Funding
- European Union's Horizon 2020 research and innovation program under the Marie Sklodowska Curie grant as part of the Print-Aid consortium [722467]
- Laboratory for Process Engineering, Environment, Biotechnology and Energy -LEPABE - FCT/MCTES (PIDDAC) [UID/EQU/00511/2019]
- Project LEPABE-2-ECO-INNOVATION - Norte Portugal Regional Operational Programme (NORTE 2020) under PORTUGAL 2020 Partnership Agreement through the European Regional Development Fund (ERDF) [NORTE-01-0145-FEDER-000005]
- BBSRC [BB/R012415/1] Funding Source: UKRI
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The lack of reproducibility of published studies is one of the major issues facing the scientific community, and the field of biofilm microbiology has been no exception. One effective strategy against this multifaceted problem is the use of minimum information guidelines. This strategy provides a guide for authors and reviewers on the necessary information that a manuscript should include for the experiments in a study to be clearly interpreted and independently reproduced. As a result of several discussions between international groups working in the area of biofilms, we present a guideline for the spectrophotometric and fluorometric assessment of biofilm formation in microplates. This guideline has been divided into 5 main sections, each presenting a comprehensive set of recommendations. The intention of the minimum information guideline is to improve the quality of scientific communication that will augment interlaboratory reproducibility in biofilm microplate assays.
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