Journal
CELL CALCIUM
Volume 91, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.ceca.2020.102255
Keywords
Mechanosensation; Mechanosensitive channel; TRP channel; TRPA1; Redox sensitivity
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Funding
- Swedish Research Council [2014-3801]
- Medical Faculty of Lund University - ALF [ALFSKANE-451751]
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The role of mammalian Transient Receptor Potential Ankyrin 1 (TRPA1) as a mechanosensor is controversial. Here, we report that purified human TRPA1 (hTRPA1) with and without its N-terminal ankyrin repeat domain responded with pressure-dependent single-channel current activity when reconstituted into artificial lipid bilayers. The hTRPA1 activity was abolished by the thiol reducing agent TCEP. Thus, depending on its redox state, hTRPA1 is an inherent mechanosensitive ion channel gated by force-from-lipids.
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