Journal
GREEN CHEMISTRY
Volume 19, Issue 22, Pages 5345-5349Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7gc02421e
Keywords
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Funding
- Royal Society [RG150021]
- Research Priority Area (RPA) of UoN in Industrial Biotechnology
- BBSRC [BB/M021947/1]
- EPSRC [EP/I033335/2]
- G. V. [PROMETEO 2016-071]
- MINECO [CTQ2015-68429R]
- MICINN [FPU13/00685]
- Biotechnology and Biological Sciences Research Council [BB/M021947/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/I033335/2] Funding Source: researchfish
- BBSRC [BB/M021947/1] Funding Source: UKRI
- EPSRC [EP/I033335/2] Funding Source: UKRI
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A method to efficiently immobilize enzymes on 3D printed continuous-flow devices is presented. Application of these chemically modified devices enables rapid screening of immobilization mechanisms and reaction conditions, simple transfer of optimised conditions into tailored printed microfluidic reactors and development of continuous-flow biocatalytic processes. The bioreactors showed good activity (8-20.5 mu mol h(-1) mg(enz)(-1)) in the kinetic resolution of 1-methylbenzylamine, and very good stability (ca. 100 h under flow).
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