Journal
GREEN CHEMISTRY
Volume 19, Issue 19, Pages 4660-4665Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7gc01696d
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Funding
- Biotechnology and Biological Sciences Research Council [BBSRC BB/M028631/1]
- Innovate UK (Biome Bioplastics)
- Royal Society
- Biotechnology and Biological Sciences Research Council [BB/M028631/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/K039687/1] Funding Source: researchfish
- BBSRC [BB/M028631/1] Funding Source: UKRI
- EPSRC [EP/K039687/1] Funding Source: UKRI
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By manipulating the reaction conditions, furandicarboxylic acid (FDCA) was prepared by the biooxidation of hydroxymethyl furfural (HMF) in a continuous one-pot reaction using galactose oxidase M3-5, periplasmic aldehyde oxidase (PaoABC), catalase and horseradish peroxidase. In addition, PaoABC was successfully entrapped in a SiO2 hydrogel and recycled 14 times without loss of activity. The catalyst was able to tolerate up to 200 mM DFF concentration giving FDCA in full conversion with very promising TOF and TON values.
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