Journal
GRASAS Y ACEITES
Volume 68, Issue 2, Pages -Publisher
CONSEJO SUPERIOR INVESTIGACIONES CIENTIFICAS-CSIC
DOI: 10.3989/gya.0109171
Keywords
Enzymatic hydrolysis; Kinetic; Lipozyme TLIM; Phoenix tree seed oil; Response surface methodology; Sterculic acid
Categories
Funding
- Program for Science & Technology Innovation Talents in Universities of Henan Province [15HASTIT30]
- Funding Scheme for Young Teachers Cultivating Program in Henan University of Technology
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Phoenix tree (Firmiana simplex) seed oil is a novel oil which is rich in sterculic acid. Sterculic acid, a cyclopropene fatty acid, can be used as the inhibitor of the stearoyl-CoA desaturase system and mammary carcinomas growth. In this work, Lipozyme TLIM-catalyzed hydrolysis of the novel Phoenix tree seed oil was used to prepare sterculic acid. High temperature GC-FID and the degree of hydrolysis (DH) were used to monitor the reaction progress. Effects of reaction variables on the hydrolysis were evaluated and optimized using response surface methodology. Results showed that sterculic acid can be successfully prepared from the novel seed oil, and the effect of reaction variables on the hydrolysis decreased in the order of reaction time > enzyme load > temperature. A high yield of fatty acids (DH, 98.2 +/- 0.8%) can be obtained under optimized conditions (45 degrees C, mass ratio of water to oil 10: 1, enzyme load 10%, and 18 h). The Arrhenius equation for the hydrolysis was LnV(0) = 9.12-4721/T. The activation energy was 39.25KJ/mol. The kinetic values for Vmax, K/m were 0.232mol/(L.min) and 0.084 mol/L, respectively.
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