Journal
MYCOLOGY-AN INTERNATIONAL JOURNAL ON FUNGAL BIOLOGY
Volume 12, Issue 1, Pages 48-57Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/21501203.2020.1725677
Keywords
Botrytis cinerea; laccases; inducers; copper; gene expression; enzyme activity
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Funding
- CSU Postgraduate Research Scholarship
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CuSO4 induces the 63.4 kDa laccase in Botrytis cinerea by induced transcription of the BcLCC2 gene, with the highest induction of BcLCC2 transcription at a concentration of 0.6 mM CuSO4. Higher concentrations of CuSO4 inhibited fungal growth. Laccase induction was not observed in the presence of gallic acid (GA).
Laccases are one of many groups of inducible enzymes produced by the filamentous fungus, Botrytis cinerea during colonisation of host plant tissues. While the processes involved in laccase induction are not fully understood, Cupric ions (e.g. CuSO4) and gallic acid (GA) have been reported as laccase inducers. This study investigates laccases activities and the expression of three laccase genes (BcLCC1, BcLCC2, BcLCC3) in three B. cinerea isolates grown in laccase-inducing medium (LIM) supplemented with CuSO4 and GA. Laccase activity in culture filtrates with CuSO4 increased after 48 h of growth in LIM at 24 degrees C. The induction of BcLCC2 transcription was greatest at a concentration of 0.6 mM CuSO4, concentrations greater than 0.6 mM inhibited fungal growth. In contrast, no laccase induction was observed in the presence of GA. Liquid chromatography-mass spectroscopy (NanoLC ESI MS/MS) analysis confirmed the presence of a 63.4 kDa protein, the BcLCC2 isoform in the culture filtrate with 0.6 mM CuSO4. Analysis of mRNA transcripts further showed BcLCC3 was also inducible and the expression of BcLCC2 and BcLCC3 was isolate-dependent. In conclusion, CuSO4 induces a 63.4 kDa laccase in B. cinerea by induced transcription of the BcLCC2 gene.
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