Journal
JOURNAL OF DIETARY SUPPLEMENTS
Volume 18, Issue 2, Pages 132-146Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/19390211.2020.1731045
Keywords
anti-cancer; apoptosis; fermented condiment; MTT; ogiri-egusi
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Funding
- African-German Network of Excellence in Science for the Intra-Africa mobility grant
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The aqueous extract from fermented Citrullus vulgaris seeds shows potential anti-cancer properties by inhibiting cancer cell growth and inducing apoptosis.
The anti-cancer activities of many fermented foods and beverages are now scientifically established. Ogiri-egusi is a condiment prepared from fermentation of Citrullus vulgaris (melon) seeds and consumed in many countries of West Africa. Its anti-oxidative and anti-hyperlipidaemic properties have been reported. This study investigated the anti-cancer activities of the aqueous and methanolic extracts from ogiri-egusi. Cytotoxicity was investigated using the MTT and colony-formation inhibition assays while flow-cytometer based Apopercentage assay was used to quantify apoptosis in extracts-treated cervical and liver cancer and normal human fibroblast cells. The inhibitory concentration responsible for killing 50% of cells after 24 h by the aqueous extract in KMST-6, HeLa, and Hep-G2 cells were estimated at 1.610, 1.020, and 1.507 mg/mL respectively. While these values reduced with increasing incubation time in cancer cells it increased in the non-cancer cell. Furthermore, the extract significantly induced apoptosis in HeLa (97 +/- 0.18%) and Hep-G2 (73 +/- 6.73%) cells. These findings were corroborated by cells morphologic presentations and inhibition of colony formation assay. These findings suggest that the aqueous extract from fermented Citrullus vulgaris seeds might be a nutraceutical with potential anti-cancer properties.
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