Journal
GENOMICS
Volume 109, Issue 3-4, Pages 290-301Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygeno.2017.05.003
Keywords
Pear; Catharanthus roseus RLK1-like kinases; Evolution; Expression pattern; Antisense oligonucleotide; Pollen tube elongation and rupture
Funding
- National Science Foundation of China [31272119]
- Doctoral Fund of Ministry of Education of China [20120097120046, 20130097130004, 20120097110041]
- Jiangsu Province Science and Technology Support Program, China [BE2014400]
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The Catharanthus roseus RLK1-like kinase (CrRLK1L) family is involved in multiple processes during plant growth. However, little is known about CrRLK1L in the wood of the pear fruit tree Pyrus bretchneideri. In this study, 26 CrRLK1L gene members were identified in pear and were grouped into six subfamilies according to phylogenetic analyses. Evolutionary analysis indicated that recent whole genome duplication (WGD) and dispersed gene duplications may contribute to the expansion of the CrRLK1L gene family in pear. Moreover, tissue-specific expression analyses suggested that CrRLK1Ls are involved in the development of various pear tissues. Subsequent qRT-PCR analyses indicated that CrRLK1Ls might play important roles in pollen tube growth. Finally, experiments with antisense oligonucleotides (ASO) demonstrated that PbrCrRLK1L26 have functions in pollen tube elongation and that PbrCrRLK1L3 regulates pollen tube rupture. These results will be useful for elaborating the biological roles of CrRLK1Ls in pear growth and development. (C) 2017 Published by Elsevier Inc.
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