Journal
STEM CELL REVIEWS AND REPORTS
Volume 17, Issue 2, Pages 694-699Publisher
SPRINGER
DOI: 10.1007/s12015-020-10043-4
Keywords
Endothelial progenitor; Endothelial colony forming cells; ECFCs; Idiopathic pulmonary fibrosis; EPCR; Thrombomodulin; Thrombin generation assay
Funding
- PROMEX STIFTUNG FUR DIE FORSCHUNG Foundation
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This study investigated hypercoagulability states in ECFCs from patients with IPF, finding higher thrombin potential in IPF-ECFCs and decreased expression of anticoagulant proteins on ECFCs membrane. This new finding suggests a potential role of ECFC in IPF pathophysiology and thrombotic-related disorders.
Idiopathic pulmonary fibrosis (IPF) is a severe, progressive and irreversible lung disease constantly associated with a major vascular remodeling process. Endothelial colony-forming cells (ECFCs) are human vasculogenic cells proposed as a cell therapy product or liquid biopsy in vascular disorders. Since the link between IPF and thrombosis has been largely proposed, the aim of our study was to explore hypercoagulability states in ECFCs from patients with IPF. We performed Thrombin generation assay (TGA) in cord blood (CB)-ECFCs, peripheral blood (PB)-ECFCs and IPF-ECFCs. Endogenous thrombin potential and peak were higher in IPF-ECFCs compared to CB-ECFCs and PB-ECFCs. As thrombin generation in ECFCs was increased, we evaluated anticoagulant proteins expressed on ECFCs membrane and identified thrombomodulin and EPCR. We found a significant decrease of both anticoagulant proteins at membrane using flow cytometry. This study is the first to examine ECFC thrombin generation in IPF. This new finding strongly argues for a role of ECFC in IPF pathophysiology and thrombotic related disorders in IPF.
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