Journal
AQUACULTURE RESEARCH
Volume 52, Issue 3, Pages 1247-1254Publisher
WILEY
DOI: 10.1111/are.14983
Keywords
Aeromonas hydrophila; green synthesis; Gum Arabic; Pseudomonas aeruginosa; silver nanoparticles
Categories
Ask authors/readers for more resources
This study evaluated the antibacterial efficacy of Gum Arabic-based silver nanoparticles against fish bacterial pathogens. The green-synthesized silver nanoparticles exhibited efficient antibacterial properties, inhibiting the growth and biofilm formation of two bacterial strains, suggesting their potential for use in commercial antibacterial products for the aquaculture industry.
This study aimed to assess the antibacterial efficacy of Gum Arabic-based silver nanoparticles against certain fish bacterial pathogens. Silver nanoparticles were green-synthesized using Gum Arabic (AgNPs-GA), which served as a stabilizing and reducing agent. The AgNPs-GA were analysed using a UV spectrophotometer set at a wavelength of 450 nm. Transmission electron micrograph analysis showed that nearly all AgNPs-GA were sphere-shaped and 10.0 nm in diameter. Dynamic light scattering (DLS) analysis showed a narrow size distribution curve, whose highest peak was at 26.2 nm. The particles were negatively charged (-17.1 +/- 4.9 mV). Silver concentration measured by inductively coupled plasma optical emission spectrometry (ICP-OES) and found to be 104 mu g/ml. The in vitro antibacterial activity of AgNPs-GA was tested against Aeromonas hydrophila and Pseudomonas aeruginosa. Gum Arabic-silver nanoparticles exhibited clear inhibition zones of 22 and 20 mm against A. hydrophila and P. aeruginosa, with a minimum inhibitory concentration of 1.625 mu g/ml and 3.25 mu g/ml for AgNPs-GA respectively. The antibiofilm activity indicated that AgNPs-GA significantly inhibit A. hydrophila and P. aeruginosa biofilm formation at AgNPs-GA concentrations of 1.625 mu g/ml. There was no significant difference at a AgNPs-GA concentration of 0.8215 mu g/ml between the control-positive groups and the AgNPs-GA treatment groups in both bacterial strains. In summary, green-synthesized sliver nanoparticles display efficient antibacterial properties, which suggests that they would be suitable for use in a commercialized antibacterial product for the aquaculture industry.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available