Journal
PLANT CELL TISSUE AND ORGAN CULTURE
Volume 144, Issue 2, Pages 485-490Publisher
SPRINGER
DOI: 10.1007/s11240-020-01963-7
Keywords
Water caltrop; Agrobacterium rhizogenes; Biolistics; In vitro; Browning; Chloroplasts
Funding
- Russian Science Foundation [18-74-00056]
- Russian Science Foundation [18-74-00056] Funding Source: Russian Science Foundation
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In this study, protocols for Agrobacterium-mediated and biolistic transformation of Trapa natans to induce hairy roots have been developed for the first time. All induced roots contained chloroplasts, showing a novel approach for genetic transformation and potential use of green photosynthesizing hairy root cultures in bioactive substance production and phytoremediation.
Hairy root cultures are often used to produce valuable metabolites. They are grown on sucrose-rich medium, which is highly susceptible to contamination. Trapa natans is a unique plant with photosynthesizing roots. It is a promising object to obtain photoautotrophic hairy root culture. Protocols for transformation of this species are yet unknown. We report that hairy roots can be induced in aquarium and in vitro cultures of T. natans by agrobacterium-mediated and biolistic transformation. 64 roots were induced by Agrobacterium rhizogenes strain 15834, two roots were obtained using strain K599. Strain A4 was not effective. Biolistics with either amplicons of rol genes and 1301 pCAMBIA plasmid carrying rol genes resulted in the formation of six roots. All these roots contained chloroplasts. This achievement opens a prospect for genetic transformation of T. natans and use of its green photosynthesizing hairy root cultures in production of bioactive substances and in phytoremediation. Key message Protocols for the Agrobacterium-mediated and biolistic transformation of Trapa natans to induce hairy roots have been developed for the first time. All induced roots contained chloroplasts.
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