Journal
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
Volume 1487, Issue 1, Pages 43-55Publisher
WILEY
DOI: 10.1111/nyas.14518
Keywords
T cells; immobilization; bone; spinal cord injury; unloading; RANKL; osteoprotegerin
Categories
Funding
- New York State Department of Health [C32093GG-3450000]
- Department of Veterans Affairs Rehabilitation Research and Development Service [B2020C]
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In mice with T cell deficiency, immobilization following spinal cord injury led to marked reduction in bone volume and more severe deterioration of bone structure compared to wild-type mice.
Whether T cells promote bone loss following immobilization after spinal cord injury (SCI) remains undetermined. Therefore, wild-type (WT) and T cell-deficient (Tcrb(-/-)) male mice underwent sham or contusion SCI to cause hindlimb paralysis. Femurs were isolated and distal and midshaft regions were evaluated by microcomputed tomography scanning. Bone marrow (BM) levels of bone turnover markers, as well as receptor activator of nuclear factor-kappa B ligand (RANKL) and osteoprotegerin (OPG), were measured by ELISA. At 2 weeks post-SCI, immobilization resulted in marked reduction in trabecular fractional bone volume (55%), thickness (40%), connectivity, and cortical thickness only in the Tcrb(-/-) animals (interaction with P < 0.05). BM analysis revealed lower bone formation (procollagen type 1 intact N-terminal propeptide), higher bone resorption (tartrate-resistant acid phosphatase-5b), and a higher RANKL/OPG ratio in the Tcrb(-/-) SCI animals. At 5 weeks post-SCI, while both WT and Tcrb(-/-) paralyzed animals showed deterioration of all indices of bone structure, they were more severe in Tcrb(-/-) animals. In summary, unlike other skeletal disorders, loss of alpha beta T cells compromises, rather than preserves, skeletal integrity under conditions of immobilization.
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