Journal
GENERAL AND COMPARATIVE ENDOCRINOLOGY
Volume 246, Issue -, Pages 309-320Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ygcen.2017.01.007
Keywords
Glycoprotein hormone; Glycoprotein receptor; Thyroid-stimulating hormone; Follicle-stimulating hormone; Luteinizing hormone; Evolution; Phylogeny; Elephant shark
Categories
Funding
- University of Oregon
Ask authors/readers for more resources
Glycoprotein hormones (GpH) interact very specifically with their receptors to mediate hypothalamic pituitary-peripheral gland endocrine signaling. Vertebrates typically have three functionally distinct GpH endocrine signaling complexes: follicle-stimulating hormone, luteinizing hormone, and thyroid stimulating hormone, and their receptors. Each hormone consists of a common alpha subunit bound to one of three different beta subunits. Individual hormone subunits and receptors are present in genomes of early metazoans, and a subset of hormone subunits and receptors has been recently characterized in sea lamprey. However, it remains unclear when the full complement of hormone and receptor protein families first appeared, and when specificity of interactions between GpH hormones and receptors first evolved. Here we present phylogenetic analyses showing that the elephant shark (Callorhinchus milii) genome contains sequences representing the current diversity of all hormone subunits and receptors in these co-evolving protein families. We examined specificity of hormone and receptor interactions using functional assays testing reporter gene activation by elephant shark follicle-stimulating hormone, luteinizing hormone, and thyroid-stimulating hormone receptors. We show highly specific, dose responsive hormone interactions for all three complexes. Our results suggest that co-evolution of specificity between proteins in these endocrine signaling complexes occurred prior to the divergence of Chondrichthyes from the chordate lineage. (C) 2017 The Authors. Published by Elsevier Inc.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available