Journal
PLANT CELL TISSUE AND ORGAN CULTURE
Volume 144, Issue 2, Pages 435-447Publisher
SPRINGER
DOI: 10.1007/s11240-020-01968-2
Keywords
Apple; bHLH transcription factor; MdPIF3; Cold stress; Drought stress
Funding
- National Key R&D Program of China [2018YFD1000200]
- National Natural Science Foundation of China [31901988, U1706202]
- Science and Technology Program of Yunnan Province [2019ZG002-1-03]
- Ministry of Agriculture of China [CARS-27]
- China Postdoctoral Science Foundation [2019M662413]
- Shandong Postdoctoral Innovation Project [201902042]
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MdPIF3, a bHLH-type protein, plays a key role in cold and drought tolerance in plants.
PHYTOCHROME INTERACTING FACTORs (PIFs) are a subset of helix-loop-helix (bHLH) transcription factors, which play critical roles in plant growth and development, as well as in adaption to ambient environments. However, PIF members have not been completely identified in apple (Malus domestica), a widely distributed fruit crop with significant economical importance. Here, we characterized MdPIF3, the homolog of AtPIF3, and determined its role in response to abiotic stresses in apple. We first analyzed its gene and protein structure, and found that it contained bHLH domain, active phytochrome B binding (APB) motif, as well as active phytochrome A binding (APA) motif. Yeast-two-hybrid assays indicated that MdPIF3 formed a homodimer by itself and heterodimers with other MdPIFs. Moreover, MdPIF3 was responsive to light and cold treatment at both transcriptional and post-translational levels. Overexpression of MdPIF3 reduced cold tolerance but enhanced drought resistance in both apple callus and Arabidopsis. Key message The bHLH-type protein, MdPIF3, plays a key role in cold and drought tolerance in plants.
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