Gold nanorods conjugated upconversion nanoparticles nanocomposites for simultaneous bioimaging, local temperature sensing and photothermal therapy of OML-1 oral cancer cells

The major challenge in photothermal therapy (PTT) is to develop nanocomposites that simultaneously exhibit bioimaging and PTT under a single near-infrared (NIR) irradiation with high therapeutic efficiency. Herein, we present a multifunctional nanocomposite synthesized by linking NaYF4:Yb3+,Er3+ upconversion nanoparticles (UCNPs) with gold nanorods (AuNR) to exhibit fluorescence labeling, local temperature sensing and photothermal functions simultaneously with a single NIR laser excitation. The AuNR-NaYF4:Yb3+,Er3+ nanocomposite particles displayed better photothermal properties compared with pure AuNRs or a blend of AuNRs and NaYF4:Yb3+,Er3+ UCNPs. The temperature-dependent upconversion luminescence (UCL) property was used to determine local temperature at the nanocomposite particles, which is useful for selecting appropriate irradiation dosage for PTT. The therapeutic performance of the nanocomposites in PTT for OML-1 oral cancer cells was determined. For cell labeling, we successfully labeled streptavidin-linked nanocomposite particles on the surface of OML-1 oral cancer using anti-human epidermal growth factor receptor 2 (anti-Her2) antibody. Finally, the nanocomposite particles caused exceptional destruction of cancer cells up to 70% dead cells under 976 nm laser irradiation for only one min at 0.3 W/cm(2) which is below the maximal permissible exposure of human skin.

Automated summary

In this study, a multifunctional nanocomposite was developed for effective treatment of oral cancer cells, demonstrating fluorescence labeling, temperature sensing, and photothermal functions. The temperature-dependent upconversion luminescence property was utilized to determine local temperature, and the nanocomposite particles successfully labeled cancer cells on the surface.