Journal
BIOPROCESS AND BIOSYSTEMS ENGINEERING
Volume 44, Issue 5, Pages 985-994Publisher
SPRINGER
DOI: 10.1007/s00449-020-02449-8
Keywords
S; aureus; Food borne pathogens; Toxins; Detection; PCR
Funding
- National Key R&D Program of China [2016YFD0400203]
- YangFan Innovative and Entrepreneurial Research Team Project [2014YT02S029]
- National Key Research and Development Program of China [2016YFD04012021]
- Fundamental Research Funds for the Central Universities [D2191310]
- AEIC [KEO-2019-0624-001-1]
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Staphylococcus aureus is an important human pathogen that can cause various life-threatening diseases. Rapid and accurate detection of virulence genes in S. aureus is crucial for preventing outbreaks caused by this pathogen. PCR is a useful tool for detecting foodborne pathogens.
Staphylococcus aureus (S. aureus) is an important human pathogen causing a variety of life-threatening diseases. In recent years, the health problem caused by S. aureus contaminated food has become a global health problem. S. aureus can express various pathogenic factors, mainly used for adhesion, colonization, invasion and infection of the host. Therefore, rapid and accurate detection of virulence genes in S. aureus is necessary to prevent outbreaks caused by this pathogen. PCR is a useful tool for rapid detection of foodborne pathogens. The objective of this study was to detect the presence of major toxin genes in S. aureus, including sea, seb, sec, see, pvl and tsst, by using a PCR plate. Of the 13 strains tested, 12 (92.3%) were found to be positive for one or more toxin genes. This study realized the one-step detection of main toxin factors in S. aureus.
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