Short tandem repeat profiling for the authentication of cancer stem-like cells

Colorectal and glioblastoma cancer stem-like cells (CSCs) are essential for translational research. Cell line authentication by short tandem repeat (STR) profiling ensures reproducibility of results in oncology research. This technique enables to identify mislabeling or cross-contamination of cell lines. In our study, we provide a reference dataset for a panel of colorectal and glioblastoma CSCs that allows authentication. Each cell line was entered into the cell Line Integrated Molecular Authentication database 2.1 to be compared to the STR profiles of 4485 tumor cell lines. This article also provides clinical data of patients from whom CSCs arose and data on the parent tumor stage and mutations. STR profiles and information of our CSCs are also available in the Cellosaurus database (ExPASy) as identified by unique research resource identifier codes.

Automated summary

Colorectal and glioblastoma cancer stem-like cells are crucial for translational research, and their authentication through STR profiling ensures research reproducibility. This study provides a reference dataset for a panel of CSCs, allowing comparison with a large number of tumor cell lines, along with clinical data and mutation information. The STR profiles and information of CSCs are available in databases for further research.