Biological Applications of Synthesized ZnO Nanoparticles Using Pleurotus djamor Against Mosquito Larvicidal, Histopathology, Antibacterial, Antioxidant and Anticancer Effect

The present study pertained to biosynthesis, characterization and biomedical application (larvicidal, histopathology, antibacterial, antioxidant and anticancer activity) of Zinc oxide nanoparticles (ZnONPs) from Pleurotus djamor. The synthesized NPs were characterized using spectral and microscopic analyses and further confirmed by UV-Visible spectrophotometer with apeak of 350 nm. The ZnONPs showed strong antioxidant property (DPPH, H2O2 and ABTS(+) radical assay) and expressed good larval toxicity against Ae. aegypti and Cx. quinquefasciatus (IVth instar larvae) with the least LC50 and LC90 values (10.1, 25.6 and 14.4, 31.7 mg/l) after 24 h treatment, respectively. We noticed the morphological changes (damaged anal papillae area and the cuticle layers) in the treated larvae. For the antibacterial assay, the highest growth inhibition zone was recorded in C. diphteriae (28.6 +/- 0.3 mm), followed by P. fluorescens (27 +/- 0.5 mm) and S. aureus (26.6 +/- 1.5 mm). The in vitro cytotoxicity assay depicted a significant level of cytotoxic effects (LC50 values 42.26 mu g/ml) of ZnONPs against the A549 lung cancer cells, even at low dose. The overall findings of the study suggest that P. djamor had the ability for the biosynthesis of ZnONPs and could act as an alternative biomedical agent for future therapeutic applications in medical avenues.

Automated summary

The study focused on biosynthesis, characterization, and biomedical application of Zinc oxide nanoparticles from Pleurotus djamor. The nanoparticles exhibited strong antioxidant, larvicidal, antibacterial, and anticancer activities, with low LC50 and LC90 values against mosquito larvae and significant cytotoxic effects on lung cancer cells. The overall findings suggest the potential of Pleurotus djamor as an alternative biomedical agent for future therapeutic applications.