An electrochemical immunosensor based on electrospun carbon nanofiber mat decorated with gold nanoparticles and carbon nanotubes for the detection of breast cancer

The aim of this work was to design and develop an electrochemical immunosensor based on electrospun carbon nanofiber (ECNF) mat for the detection of human epidermal growth factor receptor 2 (Her-2). First, ECNF mat was fabricated by electrospinning method and heat-treatment and used directly as a novel electrode. To determine Her-2, this ECNF mat electrode was modified by Au nanparticles (NPs), cysteamine (Cys) molecules, carbon nanotubes (CNTs), and antibody (Ab) molecules, respectively. Field emission scanning electron microscopy (FESEM) and energy-dispersive X-ray spectroscopy (EDS) analysis were used to characterize Au NPs modified ECNF mat electrode. In addition, analysis of Her-2 in spiked human blood serum samples were investigated by cyclic voltammetry (CV) using [Fe(CN)(6)](-3/-4) as a redox probe and the experiments were compared using commercial kit. The linear response range of the developed immunosensor was from 5 to 80 ng ml(-1) with a limit of detection (LOD) of 0.45 ng ml(-1). The results indicated that the designed immunosensor has high potential for the determination of Her-2 due to non-invasive, precise and rapid analysis.

Automated summary

This study successfully designed and developed an electrochemical immunosensor based on ECNF matrix for detecting human epidermal growth factor receptor 2. The modified ECNF matrix electrode showed a linear response range of 5 to 80 ng ml(-1) and a limit of detection of 0.45 ng ml(-1), demonstrating high potential for non-invasive, precise, and rapid analysis of Her-2.