Journal
GENE
Volume 608, Issue -, Pages 66-72Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.gene.2017.01.020
Keywords
Capsicum annuum; miRNA; Fruit development; Fruit quality; Deep sequencing
Categories
Funding
- China Agriculture Research System [CARS-25-A-8]
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MicroRNAs (miRNAs) are non-coding small RNAs which play an important regulatory role in various biological processes. Previous studies have reported that miRNAs are involved in fruit development in model plants. However, the miRNAs related to fruit development and quality in hot pepper (Capsicum annuum L.) remains unknown. In this study, small RNA populations from different fruit ripening stages and different varieties were compared using next-generation sequencing technology. Totally, 59 known miRNAs and 310 novel miRNAs were identified from four libraries using miRDeep2 software. For these novel miRNAs, 656 targets were predicted and 402 of them were annotated. GO analysis and KEGG pathways suggested that some of the predicted miRNAs targeted genes involved in starch sucrose metabolism and amino sugar as well as nucleotide sugar metabolism. Quantitative RT-PCR validated the contrasting expression patterns between several miRNAs and their target genes. These results will provide an important foundation for future studies on the regulation of miRNAs involved in fruit development and quality. (C) 2017 Elsevier B.V. All rights reserved.
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