Journal
MOLECULAR NEUROBIOLOGY
Volume 58, Issue 4, Pages 1330-1344Publisher
SPRINGER
DOI: 10.1007/s12035-020-02197-6
Keywords
microRNA; miR-210; Neuronal plasticity; Dendritic branching; Behavioural flexibility; Hypoxia
Categories
Funding
- Australian Research Council [ARC: DP120104117]
- ARC Future Fellowships [FT110100292, FT140101327]
- Australian Government Research Training Program Stipend
- Australian Research Council [FT140101327] Funding Source: Australian Research Council
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MiR-210 plays a critical role in neuroplasticity and cognitive function, with its loss in neurons leading to higher oxidative phosphorylation and ROS production, increased dendritic arbour density, and altered behavioral flexibility in cognitive tasks.
MicroRNAs are known to be critical regulators of neuronal plasticity. The highly conserved, hypoxia-regulated microRNA-210 (miR-210) has been shown to be associated with long-term memory in invertebrates and dysregulated in neurodevelopmental and neurodegenerative disease models. However, the role of miR-210 in mammalian neuronal function and cognitive behaviour remains unexplored. Here we generated Nestin-cre-driven miR-210 neuronal knockout mice to characterise miR-210 regulation and function using in vitro and in vivo methods. We identified miR-210 localisation throughout neuronal somas and dendritic processes and increased levels of mature miR-210 in response to neural activity in vitro. Loss of miR-210 in neurons resulted in higher oxidative phosphorylation and ROS production following hypoxia and increased dendritic arbour density in hippocampal cultures. Additionally, miR-210 knockout mice displayed altered behavioural flexibility in rodent touchscreen tests, particularly during early reversal learning suggesting processes underlying updating of information and feedback were impacted. Our findings support a conserved, activity-dependent role for miR-210 in neuroplasticity and cognitive function.
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