Journal
JOURNAL OF BIOPHOTONICS
Volume 14, Issue 2, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jbio.202000347
Keywords
capsaicin‐ induced calcium flow; mitochondria; nociception; photobiomodulation; sensory neurons; TRPV1
Categories
Funding
- Institute for Maternal and Child Health, IRCCS Burlo Garofolo/Italian Ministry of Health [RC02/20, RC15/17]
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This study found that PBMT at 800 and 970 nm wavelengths has a positive effect on nociceptive sensory neuronal cell line by reducing capsaicin-induced calcium flow, increasing ATP, and MMP hyperpolarization. Additionally, the 800 nm wavelength also increased ROS and O-2(-) levels.
The analgesic properties of photobiomodulation therapy (PBMT) have been raising increasing interest in the clinical community due to the positive effects observed on patients, nevertheless the mechanistic basis of its action on peripheral sensory neurons remains still elusive. In this study, the effect of near-infrared (NIR) PBMT at 800 and 970 nm of wavelength was investigated on the 50B11 immortalized nociceptive sensory neuronal cell line by evaluating capsaicin-induced calcium flow and different markers correlated to mitochondria, that is, ATP, reactive oxygen species (ROS), and mitochondrial membrane potential (MMP). Calcium peak stimulated by capsaicin, the ligand of TRPV1 channel, was decreased in neurons pre-irradiated with the combination of the two wavelengths. Furthermore, delivering the 800 and 970 nm separately an increment of ATP, as well as MMP hyperpolarization were detected; notably, the 800 nm wavelength also increased ROS and O-2(-) levels. Our findings, obtained on an in vitro model of nociception, show the positive effect of PBMT on two potential photo-targets of NIR light, namely the TRPV1 channel and the mitochondria.
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