Journal
BIO-DESIGN AND MANUFACTURING
Volume 4, Issue 1, Pages 10-21Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s42242-020-00110-7
Keywords
Reactors; Calcification; Constant composition reactor; Heart valve; In vitro; Mechanical load; Tissue engineering
Categories
Funding
- People Program (Marie Curie Actions) of the European Union's Seventh Framework FP7/2007-2013/under REA [317512]
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This study presents a comparative methodology for investigating calcium phosphate deposits on different heart valve prostheses in vitro. It was found that decellularized porcine aortic valves showed slightly higher rates of calcification compared to glutaraldehyde-fixed ones, attributed to tissue modifications from the decellularization process. Octacalcium phosphate crystallites were preferentially deposited in high mechanical stress areas, indicating the importance of mechanical forces in tissue mineralization. The in vitro circulatory model developed in this study serves as a valuable pre-screening tool for understanding the calcification process of bioprosthetic and tissue-engineered valves under physiological mechanical load.
The lifespan of biological heart valve prostheses available in the market is limited due to structural alterations caused by calcium phosphate deposits formed from blood plasma in contact with the tissues. The objective of this work is to present a comparative methodology for the investigation of the formation of calcium phosphate deposits on bioprosthetic and tissue-engineered scaffolds in vitro and the influence of mechanical forces on tissue mineralization. Based on earlier investigations on biological mineralization at constant supersaturation, a circulatory loop simulating dynamic blood flow and physiological pressure conditions was developed. The system was appropriately adapted to evaluate the calcification potential of decellularized (DCV) and glutaraldehyde-fixed (GAV) porcine aortic valves. Results indicated that DCV calcified at higher, statistically nonsignificant, rates in comparison with GAV. This difference was attributed to the tissue surface modifications and cell debris leftovers from the decellularization process. Morphological analysis of the solids deposited after 20 h by scanning electron microscopy in combination with chemical microanalysis electron-dispersive spectroscopy identified the solid formed as octacalcium phosphate (Ca-8(PO4)(6)H-2 center dot 5H(2)O, OCP). OCP crystallites were preferentially deposited in high mechanical stress areas of the test tissues. Moreover, GAV tissues developed a significant transvalvular pressure gradient increase past 36 h with a calcium deposition distribution similar to the one found in explanted prostheses. In conclusion, the presented in vitro circulatory model serves as a valuable prescreening methodology for the investigation of the calcification process of bioprosthetic and tissue-engineered valves under physiological mechanical load.
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