Journal
JOURNAL OF CANCER
Volume 12, Issue 3, Pages 630-643Publisher
IVYSPRING INT PUBL
DOI: 10.7150/jca.47278
Keywords
Pyruvate dehydrogenase kinase 1; ovarian cancer; tumor metabolic; biological behavior; prognostic biomarker
Categories
Funding
- National Natural Science Foundation of China [81772779]
- Professionals from Six-Pronged Top-Talent Program of Jiangsu Province [LGY2017068]
- The Six Top Talent Project of Jiangsu Province [2015-WSN-034]
- Medical Talent of Empowering Medicine through Science and Education Program of Jiangsu Province [ZDRCA2016003]
- Key Laboratory for Medicine of Jiangsu Province of China [ZDXKB2016005]
- Jiangning Science and Technology Development program [2019SHSY0032]
Ask authors/readers for more resources
PDK1 is highly expressed in ovarian cancer and correlates with patient survival and clinical features, serving as a potential prognostic biomarker and antineoplastic target for ovarian cancer.
Ovarian cancer (OC) is the most lethal of gynecological tumors in women. Tumor metabolism has become a new opportunity in the treatment of tumors. Pyruvate dehydrogenase kinase 1 (PDK1), as a key regulatory enzyme implicated in metabolic reprogramming of tumors, abnormally high expressed in various tumors and involved in the regulation of tumor cell biological behavior. However, the role of PDK1 in the occurrence and development of ovarian cancer remains unclear. Our team identified the expression of PDK1 in ovarian cancer cell lines and tissues through RT-PCR and immunohistochemical staining and evaluated the correlation of PDK1 expression with clinicopathologic features of patients and survival analyses. We used a variety of in vitro experiments to explore the influence of PDK1 on proliferation, invasion, migration, colony formation, apoptosis and the cell cycle of ovarian cancer cell lines CAOV3 and SKOV3. PDK1 was highly expressed in ovarian cancer cell lines and OC tissues. High expression of PDK1 was closely correlated to tumor size, FIGO stage, extraovarian metastases status and distribution. Univariate and multivariate Cox regression analysis identified that PDK1 was an independent prognostic factor for overall survival. Moreover, PDK1 was a superior predictor in prognosis of ovarian cancer and the incorporation of CA125 into PDK1 generated a predictive combination that displayed better predictive accuracy for overall survival. Downregulation of PDK1 suppressed the biological behavior of ovarian cancer cells due to S phase arrest and cellular apoptosis. PDK1 may serve as a novel prognostic biomarker, even a promising antineoplastic target of ovarian cancer.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available