4.5 Article

circHIPK3 promotes proliferation and migration and invasion via regulation of miR-637/HDAC4 signaling in osteosarcoma cells

Journal

ONCOLOGY REPORTS
Volume 45, Issue 1, Pages 169-179

Publisher

SPANDIDOS PUBL LTD
DOI: 10.3892/or.2020.7833

Keywords

osteosarcoma; circular RNA HIPK3; microRNA-637; migration and invasion; proliferation

Categories

Funding

  1. Key R&D Program of Liaoning Province [20180550251]

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The study revealed that circHIPK3 was upregulated in osteosarcoma and promoted cell proliferation, migration, and invasion by sponging miR-637 to increase HDAC4 expression. This circHIPK3/miR-637/HDAC4 axis plays a crucial role in the progression of osteosarcoma.
Accumulating evidence has indicated that circular RNAs (circRNAs) serve crucial roles in the progression of a diverse range of different types of cancer, including osteosarcoma (OS). The present study determined the expression pattern and function of circRNA homeodomain interacting protein kinase 3 (circHIPK3), a novel circular RNA, in OS. It was revealed that circHIPK3 expression was upregulated in OS tissue samples and OS cell lines. A localization assay revealed that circHIPK3 was primarily located in the cytoplasm. Using loss-of-function proliferation and Transwell assays, the present study revealed that circHIPK3-knockdown suppressed OS cell proliferation, migration and invasion. Furthermore, the present study screened potential microRNAs that may interact with circHIPK3. It was revealed that microRNA-637 (miR-637) expression was downregulated in OS according to a Gene Expression Omnibus data analysis. In addition, the present study demonstrated that miR-637 expression was downregulated in OS cell lines. A fluorescence in situ hybridization assay revealed that both miR-637 and circHIPK3 were located in the cytoplasm. An in-depth mechanism investigation demonstrated that circHIPK3 expression was inversely correlated with miR-637 expression, and that circHIPK3 was a target of miR-637. In addition, it was revealed that histone deacetylase 4 (HDAC4) was another downstream target gene of miR-637, as demonstrated using a luciferase assay. It was revealed that miR-637 suppressed OS cell proliferation, migration and invasion via targeting of HDAC4. Finally, the present study demonstrated that circHIPK3 sponged miR-637 to promote HDAC4 expression and OS cell proliferation, migration and invasion. In conclusion, the present study uncovered the role of the circHIPK3/miR-637/HDAC4 axis in OS cell proliferation, migration and invasion. It was demonstrated that circHIPK3 promoted OS cell proliferation, migration and invasion by modulating miR-637/HDAC4 signaling.

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