Journal
BIORESOURCE TECHNOLOGY
Volume 320, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.actatropica.2020.124290
Keywords
Long chain fatty acid; Differential pulse voltammetry; Multi-heme Cytochrome C; Exciton coupling; Circular dichroism
Funding
- Japan Society for Promotion of Science KAKENHI [17H04969]
- PRIME
- Japan Agency for Medical Research and Development [19gm6010002h0004]
- JST, PRESTO, Japan [JPMJPR19H1]
- Japan Society for Promotion of Science [P20105]
Ask authors/readers for more resources
Alteration of outer membrane composition in Shewanella oneidensis MR-1 significantly enhanced the electron flow rate via cell-surface redox protein MtrC. Culturing at lower temperatures increased the rate of extracellular electron transfer, suggesting that modification of membrane composition may be a novel strategy for improving the efficiency of EET-based technologies.
While cell membrane composition is critical for the function of membrane proteins, membrane modification has not been used to control the rate of extracellular electron transfer (EET) via the outer membrane protein complexes. Here, the rate of electron flow via the cell-surface redox protein, MtrC, was highly enhanced upon change in the outer membrane composition in Shewanella oneidensis MR-1. The MR-1 strain was pre-cultured at 4 degrees C and 30 degrees C to initiate differentiation of membrane composition. The whole-cell difference electrochemical assay of wild-type and mutant strains lacking MtrC suggested that the rate of EET via MtrC increased approximately 18 times at 4 degrees C than 30 degrees C. Circular dichroism spectroscopy showed that the molar exciton coupling coefficient for inter-heme interaction in MtrC increased in MR-1 at 4 degrees C than 30 degrees C. Results suggest that membrane modification may be a novel strategy for improving the efficiency of EET-based technologies.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available