Journal
STEM CELL REVIEWS AND REPORTS
Volume 17, Issue 3, Pages 1027-1038Publisher
SPRINGER
DOI: 10.1007/s12015-020-10089-4
Keywords
Mesenchymal stem cell; Exosomes; Triple negative breast cancer; miR-381; WNT; EMT
Funding
- School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences,Tehran, Iran [11985]
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This study demonstrated that ADMSC-exosomes efficiently delivered miR-381 mimic to MDA-MB-231 cells, leading to significant inhibition of proliferation, migration, and invasion capabilities of triple-negative breast cancer cells, while promoting apoptosis. This suggests that ADMSC-exosomes have the potential to be effective nanocarriers for RNA-based therapies.
Recent investigations have emphasized the role of aberrant expression of microRNAs (miRNAs) in progression of almost all types of cancers. Exosomes, membrane-enclosed natural nanovesicles, transport cellular contents, including proteins, mRNAs, and miRNAs, between cells. Unique features of exosomes make them an appropriate carrier for drug delivery. miRNA-381 is one of the downregulated miRNAs in several cancers including triple-negative breast cancer (TNBC) and restoration of its expression in TNBC cells can restrict their migratory ability through targeting several signaling pathways. In current study, we exploited the exosomes isolated from adipose-derived mesenchymal stem cells (ADMSC-exosomes) to deliver miR-381 mimic to MDA-MB-231 cells to elucidate their effects on TNBC cells. The effects of miR-381 loaded ADMSC-exosomes on proliferation, apoptosis, migration, and invasion of MDA-MB-231 cells were analyzed. Our results indicated that ADMSC-exosomes were successfully isolated and internalized by MDA-MB-231 cells. miR-381 mimic was efficiently delivered to MDA-MB-231 cells by ADMSC-exosomes. miR-381 loaded ADMSC-exosomes significantly downregulated the expression of epithelial to mesenchymal transition (EMT) related genes and proteins. Notably, miR-381 loaded ADMSC-exosomes inhibited proliferation, migration, and invasion capacity of MDA-MB-231 and promoted their apoptosis in vitro. Taken together, we showed that ADMSC-exosomes could be used as efficient nanocarriers for RNA-based therapies.
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