Journal
THERANOSTICS
Volume 11, Issue 4, Pages 1991-2005Publisher
IVYSPRING INT PUBL
DOI: 10.7150/thno.50613
Keywords
collagen; fibronectin; glioma; PI3K/AKT; CDC42
Categories
Funding
- Project Program of Neurosurgical Clinical Medical Research Center of Sichuan Province
- Science and Technology Support Project of Sichuan Province [2018JY0404]
- Science and Technology Foundation of Southwest Medical University [2017-ZRQN180, 2017-ZRQN-110]
Ask authors/readers for more resources
Cancer development, especially in glioma, involves complex interactions between extracellular matrix components, such as type I collagen and fibronectin, and signaling pathways like PI3K/AKT/SOX2 and CDC42/YAP-1/NUPR1/Nestin. Inhibiting the integrin alpha v beta 3 pathway shows promise in suppressing tumor growth and reducing cancer relapse, providing new insights for potential glioma therapy strategies.
Cancer development is a complex set of proliferative progression, which arises in most cases via multistep pathways associated with various factors, including the tumor microenvironment and extracellular matrix. However, the underlying mechanisms of cancer development remain unclear and this study aimed to explore the role of extracellular matrix in glioma progression. Methods: The expression of type I collagen and fibronectin in tumor tissues from glioma patients was examined by immunofluorescence staining. The correlations between collagen/fibronectin and glioma progression were then analyzed. A 3D collagen/fibronectin cultured system was established for tumor cells culture in vitro. Quantitative, real-time PCR and western blot were used to detect PI3K/ATK and CDC42 signals associated proteins expression in glioma. We used in vitro Cell Counting Kit-8, colony formation, and tumorigenesis assays to investigate the function of PI3K/AKT and CDC42 signals associated proteins. A xenograft glioma mice model was also used to study the anticancer effects of integrin inhibitor in vivo. Results: Our study demonstrated that type I collagen and fibronectin collaborate to regulate glioma cell stemness and tumor growth. In a 3D collagen/fibronectin culture model, glioma cells acquired tumorigenic potential and revealed strengthened proliferative characteristics. More significantly, collagen/fibronectin could facilitate the activation of PI3K/AKT/SOX2 and CDC42/YAP-1/NUPR1/Nestin signaling pathways via integrin alpha v beta 3, eliciting sustained tumor growth and cancer relapse. Combination of the integrin signaling pathway inhibitor and the chemotherapeutic agent efficiently suppressed glioma cell proliferation and tumorigenic ability. Conclusion: We demonstrated that type I collagen and fibronectin could collaborate to promote glioma progression through PI3K/AKT/SOX2 and CDC42/YAP-1/NUPR1/Nestin signaling pathways. Blockade of the upstream molecular integrin alpha v beta 3 revealed improved outcome in glioma therapy, which provide new insights for eradicating tumors and reducing glioma cancer relapse.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available