Journal
BULLETIN OF ENTOMOLOGICAL RESEARCH
Volume 111, Issue 1, Pages 57-65Publisher
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0007485320000644
Keywords
Abiotic stress; AccCYP4AV1; apis cerana cerana Fabricius; cytochrome P450 monooxygenases; RNA interference
Categories
Funding
- Shandong agricultural fine varieties breeding Projects [2017LZN006]
- National Natural Science Foundation of China [31572470]
- earmarked fund for the China Agriculture Research System [CARS-44]
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The gene AccCYP4AV1, isolated from Apis cerana cerana, shows high expression in the midgut and responds to stressors like low temperature and ultraviolet radiation. However, it is downregulated by high temperature and chemical stressors. The protein encoded by AccCYP4AV1 is believed to be involved in defense against abiotic stress damage.
Cytochrome P450 monooxygenases (P450s) play significant roles in protecting organisms from abiotic stress damage. Here, we report the sequence and characterization of a P450s gene (AccCYP4AV1), isolated from Apis cerana cerana Fabricius. The open reading frame of AccCYP4AV1 is 1506 base pairs long and encodes a predicted protein of 501 amino acids and 57.84 kDa, with an isoelectric point of 8.67. Real-time quantitative polymerase chain reaction (RT-qPCR) analysis indicated that AccCYP4AV1 is more highly expressed in the midgut than in other tissues. In addition, the highest expression occurs in newly emerged adult workers, followed by the first instar of the larval stage. In addition, the expression of the AccCYP4AV1 was upregulated by low temperature (4 degrees C), ultraviolet radiation, hydrogen peroxide, paraquat, and dichlorvos treatments. In contrast, AccCYP4AV1 transcription was downregulated by other abiotic stress conditions: exposure to increased temperature (44 degrees C), deltamethrin, cadmium chloride, and mercury (II) chloride. Moreover, when AccCYP4AV1 was knocked-down by RNA interference, the results suggested that multiple antioxidant genes (AccsHSP22.6, AccSOD2, AccTpx1, and AccTpx4) were downregulated and antioxidant genes AccGSTO1 and AccTrx1 were upregulated. The activity levels of peroxidase and catalase were upregulated in the AccCYP4AV1-knocked-down samples, compared with those in the control groups. These findings suggest that the AccCYP4AV1 protein might be involved in the defense against abiotic stress damage.
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