Biocatalytic ketone reductions using Biobeads for miniaturized high throughput experimentation

Miniaturized reactions conducted in parallel can lead to increased productivity in laboratories without depleting high value reagents. The use of polystyrene beads to coat thin layers of solid reagents offers an elegant strategy to tackle the microscale high throughput solid dispensing conundrum. Herein we report the successful utilization of Biobeads as the first example of high throughput reaction screening with nanomole quantities of ketoreductase on polystyrene beads for asymmetric biocatalytic reactions.

Automated summary

Conducting miniaturized reactions in parallel can increase laboratory productivity without depleting high value reagents. Coating thin layers of solid reagents with polystyrene beads offers an elegant strategy to address the microscale high throughput solid dispensing challenge. The successful utilization of Biobeads on polystyrene beads for high throughput reaction screening with nanomole quantities of ketoreductase demonstrates a promising approach for asymmetric biocatalytic reactions.