4.7 Article

Impacts of cotton linter pulp characteristics on the processivity of glycoside hydrolase family 5 endoglucanase from Volvariella Volvacea

Journal

CELLULOSE
Volume 28, Issue 4, Pages 1947-1959

Publisher

SPRINGER
DOI: 10.1007/s10570-020-03665-x

Keywords

Processive endoglucanase; Processivity; Degree of polymerization; Crystallinity index; Cotton linter pulp; Cellulose-reducing ends

Funding

  1. Natural Science Foundation of China [31730106, 31470593]

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The study found that the crystallinity of cotton linter pulp has the most significant impact on the processivity of EG1 enzyme, followed by the initial cellulose-reducing ends. The degree of polymerization (DP) indirectly affects enzymatic hydrolysis, influenced by the pulp preparation method and conditions.
EG1 from Volvariella volvacea is a processive endoglucanase belonging to glycoside hydrolase family 5. The impacts of cotton linter pulp characteristics, such as degree of polymerization (DP), crystallinity, and the initial cellulose-reducing ends, on the processivity of EG1 were investigated. Three commercial cotton linter pulp with different DP were used in present study. Ball milling was used to alter the crystallinity and DP of cellulose. The results indicate that the crystallinity has the most significant impact on enzyme processivity followed by initial cellulose-reducing ends. Whereas the DP indirectly affects the enzymatic hydrolysis and influenced by the pulp preparation method and conditions. The initial cellulose-reducing ends also affect enzyme adsorption but their impact is not obvious when the crystallinity is very low. These results also demonstrate the endo- and exo-action are both exist for EG1. The processive exo-action can start from the newly created cellulose-reducing ends by endo-action as well as the initial cellulose-reducing ends. The contribution of initial cellulose-reducing ends is affected by its quantity and cellulose crystallinity. A plausible action mode for EG1 is also proposed.

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