Journal
LAB ON A CHIP
Volume 21, Issue 1, Pages 113-121Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0lc00966k
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Funding
- Taiwan's Ministry of Science and Technology [MOST107-2221-E-007-013-MY3, 106-2221-E-007-029-MY3]
- Kaohsiung Chang Gung Hospital, Taiwan [CMRPG8K0501]
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The study developed an integrated microfluidic chip for rapid isolation of bacteria from blood, aiding in early sepsis diagnosis. The device is efficient with a high capture rate of 85% and the entire detection process can be completed in just 4 hours.
Since early diagnosis of sepsis may assist clinicians in initiating timely, effective, and prognosis-improving antibiotic therapy, we developed an integrated microfluidic chip (IMC) for rapid isolation of both Gram-positive and Gram-negative bacteria from blood. The device comprised a membrane-based filtration module (90 min operating time), a bacteria-capturing module using a micro-mixer containing magnetic beads coated with flexible neck regions of mannose-binding lectin proteins for bacteria capture (20 min), and a miniature polymerase chain reaction (PCR) module for bacteria identification (90 min via TaqMan (R) probe technology). The filter separated all white blood cells and 99.5% of red blood cells from bacteria, which were captured at rates approaching 85%. The PCR assay's limit of detection was 5 colony-forming units (CFU) per reaction, and the entire process was completed in only 4 h. Since this is far less than that for culture-based approaches, this IMC may serve as a promising device for detection of sepsis.
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