Cellular memory in eukaryotic chemotaxis depends on the background chemoattractant concentration

Cells of the social amoeba Dictyostelium discoideum migrate to a source of periodic traveling waves of chemoattractant as part of a self-organized aggregation process. An important part of this process is cellular memory, which enables cells to respond to the front of the wave and ignore the downward gradient in the back of the wave. During this aggregation, the background concentration of the chemoattractant gradually rises. In our microfluidic experiments, we exogenously applied periodic waves of chemoattractant with various background levels. We find that increasing background does not make detection of the wave more difficult, as would be naively expected. Instead, we see that the chemotactic efficiency significantly increases for intermediate values of the background concentration but decreases to almost zero for large values in a switch-like manner. These results are consistent with a computational model that contains a bistable memory module, along with a nonadaptive component. Within this model, an intermediate background level helps preserve directed migration by keeping the memory activated, but when the background level is higher, the directional stimulus from the wave is no longer sufficient to activate the bistable memory, suppressing directed migration. These results suggest that raising levels of chemoattractant background may facilitate the self-organized aggregation in Dictyostelium colonies.

Automated summary

Cells of the social amoeba Dictyostelium discoideum exhibit cellular memory, enabling them to respond to chemoattractant waves while ignoring downward gradients. Increasing background levels of chemoattractant can significantly enhance chemotactic efficiency, peaking at intermediate values and decreasing to near zero at high values. These results suggest that raising levels of chemoattractant background may facilitate self-organized aggregation in Dictyostelium colonies.