Highly convenient and highly specific-and-sensitive PCR using Se-atom modified dNTPs

Primer design and condition optimization for PCR are tedious and labour-intensive. To conveniently achieve high selectivity, sensitivity and robustness, herein, we first report a new strategy with Se-dNTPs to enhance PCR specificity (over 240-fold) and sensitivity (up to single-digit), effectively eliminating non-specific products and simplifing PCR design and optimization.

Automated summary

A new strategy using Se-dNTPs to enhance PCR specificity and sensitivity is reported in this study, simplifying PCR design and optimization by effectively eliminating non-specific products.