Journal
NATURE METABOLISM
Volume 3, Issue 2, Pages 149-+Publisher
NATURE PORTFOLIO
DOI: 10.1038/s42255-021-00347-1
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Funding
- Deutsche Forschungsgemeinschaft (DFG) via 'focus funding on COVID-19' DFG [DFG KL 2544/8-1-AO 673221, 'Sachbeihilfe' KL 2544/7-1, 'Heisenberg-Programm' KL 2544/6-1]
- Baden-Wurttemberg-Foundation ExPoChip
- MWK Baden-Wurttemberg
- BMBF
- EU's Horizon 2020 research and innovation program (Fight-nCoV) [101003555]
- DFG [380319649, 376202546]
- Federal Ministry of Education and Research of Germany [01KI2014]
- Else-Kroner-Fresenius Excellence funding - International Graduate School in Molecular Medicine, Ulm
- Baden-Wurttemberg Stiftung
- Bausteinprogramm
- Excellence Initiative of the German federal and state governments
- German Center for Diabetes Research (DZD e.V.)
- Helmholtz Alliance 'Aging and Metabolic Programming
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The study shows that SARS-CoV-2 can infect and impact insulin secretion in human pancreatic cells, potentially contributing to metabolic dysregulation observed in COVID-19 patients.
Infection-related diabetes can arise as a result of virus-associated beta-cell destruction. Clinical data suggest that the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), causing the coronavirus disease 2019 (COVID-19), impairs glucose homoeostasis, but experimental evidence that SARS-CoV-2 can infect pancreatic tissue has been lacking. In the present study, we show that SARS-CoV-2 infects cells of the human exocrine and endocrine pancreas ex vivo and in vivo. We demonstrate that human beta-cells express viral entry proteins, and SARS-CoV-2 infects and replicates in cultured human islets. Infection is associated with morphological, transcriptional and functional changes, including reduced numbers of insulin-secretory granules in beta-cells and impaired glucose-stimulated insulin secretion. In COVID-19 full-body postmortem examinations, we detected SARS-CoV-2 nucleocapsid protein in pancreatic exocrine cells, and in cells that stain positive for the beta-cell marker NKX6.1 and are in close proximity to the islets of Langerhans in all four patients investigated. Our data identify the human pancreas as a target of SARS-CoV-2 infection and suggest that beta-cell infection could contribute to the metabolic dysregulation observed in patients with COVID-19. SARS-CoV-2 is shown to infect and replicate in human pancreatic tissue, including in beta-cells, which is associated with morphological, transcriptomic and functional changes.
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