Prospects for 3D bioprinting of organoids

Three-dimensional (3D) organoids derived from pluripotent or adult tissue stem cells seem to possess excellent potential for studying development and disease mechanisms alongside having a myriad of applications in regenerative therapies. However, lack of precise architectures and large-scale tissue sizes are some of the key limitations of current organoid technologies. 3D bioprinting of organoids has recently emerged to address some of these impediments. In this review, we discuss 3D bioprinting with respect to the use of bioinks and bioprinting methods and highlight recent studies that have shown success in bioprinting of stem cells and organoids. We also summarize the use of several vascularization strategies for the bioprinted organoids, that are critical for a complex tissue organization. To fully realize the translational applications of organoids in disease modeling and regenerative medicine, these areas in 3D bioprinting need to be appropriately harnessed and channelized.

Automated summary

Three-dimensional bioprinting technology is emerging to address limitations in current organoid technologies, showing success in printing stem cells and organoids. Vascularization strategies for bioprinted organoids are critical for achieving complex tissue organization. Harnessing and directing advancements in 3D bioprinting is essential for realizing the translational applications of organoids in disease modeling and regenerative medicine.