Exogenous Melatonin Improves Physiological Characteristics and Promotes Growth of Strawberry Seedlings Under Cadmium Stress

Melatonin, as a plant growth regulator, is involved in plant stress resistance. We studied the effects of different concentrations (0, 10, 50, 100, 150, and 200 mu mol.L-1) of melatonin on the growth and physiological characteristics of strawberry under cadmium (Cd) stress. The results represented that the growth of strawberry seedlings was inhibited under Cd stress, and the seedling biomass, chlorophyll content and the activities of antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) decreased. These toxic effects were, however, effectively remediated by exogenous melatonin pretreatment. Melatonin treatment slowed the inhibitory effect of seedling growth and significantly increased seedling biomass, chlorophyll content, antioxidant enzyme activities, and soluble protein levels in leaves and roots compared with the control. Melatonin treatment also reduced the malondialdehyde (MDA) content and the oxidative stress reactions, increased the anthocyanin content, and slowed the senescence rate, thereby reducing the toxic effects of Cd on strawberries. This indicates that foliar application of melatonin can effectively remediate the adverse effects of Cd on strawberry seedlings; the greatest effect was obtained with melatonin concentration of 100 mu mol.L-1.

Automated summary

Melatonin, as a plant growth regulator, can effectively alleviate the inhibitory effects of Cd stress on strawberry seedlings. Treatment with exogenous melatonin increases seedling biomass, chlorophyll content, and antioxidant enzyme activities, while reducing malondialdehyde content and oxidative stress reactions. Foliar application of melatonin at a concentration of 100 mu mol.L-1 shows the greatest remediation effect on Cd toxicity in strawberries.