Journal
AGING-US
Volume 13, Issue 1, Pages 1032-1050Publisher
IMPACT JOURNALS LLC
Keywords
SPOCK1; SIX1; EMT; breast cancer; AKT/mTOR signaling pathway
Categories
Funding
- Special Funds for Local Science and Technology Development Guided by the Central Committee, basic research project of Jilin Province [202002021JC]
- National Natural Science Funds of China [31760313]
- Funds of Tumen River Scholar Project
- Key Laboratory of the Science and Technology Department of Jilin Province [20170622007JC]
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SPOCK1 plays a crucial role in breast cancer progression by promoting cell proliferation and metastasis, making it a promising clinical therapeutic target for preventing the progression of breast cancer.
SPOCK1 is highly expressed in many types of cancer and has been recognized as a promoter of cancer progression. Its regulatory mechanism in breast cancer (BC) remains unclear. This study aimed to explore the precise function of SPOCK1 in BC progression and to identify the mechanism by which SPOCK1 is involved in cell proliferation and epithelial-mesenchymal transition (EMT). Immunohistochemistry (IHC) experiments and database analysis showed that high expression of SPOCK1 was positively associated with histological grade, lymph node metastasis (LN) and poor clinical prognosis in BC. A series of in vitro and in vivo assays elucidated that altering the SPOCK1 level led to distinct changes in BC cell proliferation and metastasis. Investigations of potential mechanisms revealed that SPOCK1 interacted with SIX1 to enhance cell proliferation, cell cycle progression and EMT by activating the AKT/mTOR pathway, whereas inhibition of the AKT/mTOR pathway or depletion of SIX1 reversed the effects of SPOCK1 overexpression. Furthermore, SPOCK1 and SIX1 were highly expressed in BC and might indicate poor prognoses. Altogether, the SPOCK1/SIX1 axis promoted BC progression by activating the AKT/mTOR pathway to accelerate cell proliferation and promote metastasis in BC, so the SPOCK1/SIX1 axis might be a promising clinical therapeutic target for preventing BC progression.
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