Journal
GLYCOBIOLOGY
Volume 31, Issue 3, Pages 181-187Publisher
OXFORD UNIV PRESS INC
DOI: 10.1093/glycob/cwaa085
Keywords
glycoproteomics; mass spectrometry; SARS-CoV-2; spike glycoprotein
Categories
Funding
- Biotechnology and Biological Sciences Research Council [BB/V011324/1]
- BBSRC [BB/V011324/1] Funding Source: UKRI
Ask authors/readers for more resources
This study analyzed the glycosylation structure of the novel coronavirus SARS-CoV-2 and identified complex-type N-glycans as well as a single site of O-glycosylation within the receptor-binding domain.
The novel coronavirus SARS-CoV-2, the infective agent causing COVID-19, is having a global impact both in terms of human disease as well as socially and economically. Its heavily glycosylated spike glycoprotein is fundamental for the infection process, via its receptor-binding domains interaction with the glycoprotein angiotensin-converting enzyme 2 on human cell surfaces. We therefore utilized an integrated glycomic and glycoproteomic analytical strategy to characterize both Nand O-glycan site-specific glycosylation within the receptor-binding domain. We demonstrate the presence of complex-type N-glycans with unusual fucosylated LacdiNAc at both sites N331 and N343 and a single site of O-glycosylation on T323.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available