Isolation and Characterization of Klebsiella Phages for Phage Therapy

Introduction: Klebsiella is a clinically important pathogen causing a variety of antimicrobial resistant infections in both community and nosocomial settings, particularly pneumonia, urinary tract infection, and sepsis. Bacteriophage (phage) therapy is being considered a primary option for the treatment of drug-resistant infections of these types.Methods: We report the successful isolation and characterization of 30 novel, genetically diverse Klebsiella phages.Results: The isolated phages span six different phage families and nine genera, representing both lysogenic and lytic lifestyles. Individual Klebsiella phage isolates infected up to 11 of the 18 Klebsiella capsule types tested, and all 18 capsule-types were infected by at least one of the phages.Conclusions: Of the Klebsiella-infecting phages presented in this study, the lytic phages are most suitable for phage therapy, based on their broad host range, high virulence, short lysis period and given that they encode no known toxin or antimicrobial resistance genes. Phage isolates belonging to the Sugarlandvirus and Slopekvirus genera were deemed most suitable for phage therapy based on our characterization. Importantly, when applied alone, none of the characterized phages were able to suppress the growth of Klebsiella for more than 12 h, likely due to the inherent ease of Klebsiella to generate spontaneous phage-resistant mutants. This indicates that for successful phage therapy, a cocktail of multiple phages would be necessary to treat Klebsiella infections.

Automated summary

This study successfully isolated and characterized 30 novel, genetically diverse Klebsiella phages, belonging to six different phage families and nine genera. Among the Klebsiella-infecting phages, lytic phages were deemed most suitable for phage therapy due to their broad host range, high virulence, short lysis period, and lack of known toxin or antimicrobial resistance genes. It was found that a cocktail of multiple phages would be necessary to effectively treat Klebsiella infections due to the ease of Klebsiella to generate spontaneous phage-resistant mutants.